Blood Agar - Composition and bacteria cultured - Important Cultural Media

Blood Agar:

Composition:
1. Sterile defibrinated blood 5-10 %
2. Melted agar
3. Beef extract
4. Peptone water
5. NaCl

container:
Petri dish

Colour:
Opaque red.

Consistency:
Solid medium.

Micro-organism Cultured.
This media is used to determine the haemolytic properties of bacteria.

1. Complete (beta) hemolytic bacteria:- Streptococcus pyogenes,
2. Incomplete (alpha) hemolytic bacteria:- Streptococcus viridans, Streptococcus pneumoniae
3. No (gamma) hemolysis:- Staphylococcus Albus, staphylococcus citrus, Staphylococcus epidermidis, etc.

Important Culture Media:

a) Nutrient agar

b) Blood agar

c)Chocolate agar

d)McConkey's Medium

e) Lowenstein Jensen (LJ) medium

f) Loeffler's Coagulated Medium

g) Robertson's cooked meat medium) Tripple sugar iron (TSI) medium

i) Christensen's Urea Medium

j) Simmon Citrate Medium

k) Peptone Water

l) Nutrient Broth

m) Sugar Media