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Laboratory diagnosismicrobiology

Sugar Media - Composition and Uses - Important Culture Media




Sugar Media:

Composition:
1. Nutrient broth
2. Peptone water
3. Bromocresol purple (indicator)
4. Sugar (eg. glucose, sucrose, lactose, maltose xylose, mannitol, for which fermentation is to be determined)

Container:
Test tube.

Color.
1. Purple befor reaction
2. Yellow after reaction

consistency:
Liquid medium

Uses:
To determine which sugar is utilized by bacteria. Type of sugar contained in test tube is known by the color of cotton plug of test tube, which are.
1. glucose - green
2. sucrose- white
3. Lactose- red
4. Maltose- Blank
5. Xylose- Blue
6. Mannitol- Violet.

Important Culture Media:

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