Sugar Media - Composition and Uses - Important Culture Media

Sugar Media:

Composition:
1. Nutrient Broth
2. Peptone water
3. Bromocresol purple (indicator)
4. Sugar (eg. glucose, sucrose, lactose, maltose xylose, mannitol, for which fermentation is to be determined)

Container:
Test tube.

Colour.
1. Purple before reaction
2. Yellow after reaction

consistency:
Liquid medium

Uses:
To determine which sugar is utilized by bacteria. Type of sugar contained in a test tube is known by the colour of the cotton plug of the test tube, which are.
1. glucose - green
2. sucrose- white
3. Lactose- red
4. Maltose- Blank
5. Xylose- Blue
6. Mannitol- Violet.

Important Culture Media:

a) Nutrient agar

b) Blood agar

c)Chocolate agar

d)McConkey's Medium

e) Lowenstein jensen (LJ) medium

f) Loeffler's Coagulated Medium

g) Robertson's cooked meat medium

h) Tripple sugar iron (TSI) medium

i) Christensen's Urea Medium

j) Simmon Citrate Medium

k) Peptone Water

l) Nutrient Broth

m) Sugar Media.