Chocolate Agar - Composition-bacteria cultured-Important Culture Media

Chocolate Agar:

Composition:
its composition is the same as blood agar. that is
1. sterile defibrinated blood 5-10 %
2. Melted agar
3. Beef extract,
4. Peptone water
5. NaCl

A blood agar plate is put in boiling water for about 1 hour until its colour changes from red to chocolate brown. Heating converts Haemoglobin into hematin.

Container:
petri dish.

Colour:
Opaque Chocolate colour

Consistency:
solid medium

Micro-organism Cultured.
1. Haemophilus influenzae
2. Neisseria gonorrheae
3. Neisseria meningitidis
4. streptococcus pneumoniae.

Important Culture Media:

a) Nutrient agar

b) Blood agar

c)Chocolate agar

d)McConkey's Medium

e) Lowenstein Jensen (LJ) medium

f) Loeffler's Coagulated Medium

g) Robertson's cooked meat medium

h) Triple sugar iron (TSI) medium

i) Christensen's Urea Medium

j) Simmon Citrate Medium

k) Peptone Water

l) Nutrient Broth

m) Sugar Media.