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Laboratory diagnosismicrobiology

Chocolate Agar - Composition-bacteria cultured-Important Culture Media

Chocolate Agar:

its composition is same as blood agar. that is
1. sterile defibrinated blood 5-10 %
2. Melted agar
3. Beef extract,
4. Peptone water
5. NaCl

Blood agar plate is put in boiling water for about 1 hour until its color changes from red to chocolate brown . Heating converts Hamoglobin into hematin.

petri dish.

Opaque Chocolate color

solid medium

Micro-organism Cultured.
1. Haemophilus influenzae
2. Neisseria gonorrheae
3. Neisseria meningitidis
4. streptococcus pneumoniae.

Important Culture Media:

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